SYNTHESIS AND STUDY OF BIOLOGICALLY ACTIVE ADDITIVES FOR ANTIBACTERIAL COMPOSITION MATERIALS (56-57)

56 Национальная ассоциация ученых (НАУ) # 50 , 201 9
ХИМИЧЕСКИЕ НАУКИ

SYNTHESIS AND STUDY OF BIOLOGICALLY ACTI VE ADDITIVES FOR A NTIBACTERIAL
COMPOSITION MATERIAL S

Guliyeva Shahana Israil
Assistant of Azerbaijan Medical
University, Baku
Alikhanova Aygun Ilham
Scientist of Institute of Polymer Materials,
NAS of Azerbaijan, Sumgait

СИНТЕЗ И ИЗУЧЕНИЕ БИОЛОГИЧЕ СКИ АКТИВНЫХ ДОБАВОК ДЛЯ МАТЕРИАЛОВ
АНТИБАКТЕРИАЛЬНОЙ КО МПОЗИЦИ И

Гулиева Шахана Иcраиль
ассистент Азербайджанской Медицинско й
Университет, Баку
Алиханова Айгюн
Научн. сотрудник Института Полимерных
Материалов, НАН Азербайджана, г. Су мгаит

Abstract
In the presented article the antibacterial properties of maleimide derivatives of aminosalicylic acids were
compared. Primary ant ibacterial and antifungal derivatives of the presented preparations were studied by diffusion
method.
As a resu lt of the research, it was found that the presented maleimide derivatives of the ortho -, meta -, and
para -aminosaliciles had an active influence o n the cultures of individual concentrated microorganisms, whereas
the sterile distilled water exhibited poor an timicrobial activity. By comparison, the influence of all three substances
on fungal cells (C.albicans) was more effective.
Аннотация
В представл енном статье сравнивались антибактериальные свойства малеимидных производных
аминосалициловых кислот. Первичные антибактериальные и противогрибковые про изводные
представленных препаратов были изучены методом диффузии.
В результате проведенных исследований было обнаружено, что представленные малеимидные
производные орто -, мета - и парааминосалицилов оказывали активное влияние на культуры отдельных
концентри рованных микроорганизмов, в то время как стерильная дистиллированная вода прояв ляла
слабую антимикробную активность. Для сравнения, воздействие всех трех веществ на клетки грибов
(C.albicans) было более эффективным.
Keywords - antibacterial polymer additiv es, antibacterial composition materials, cyclic imides, antimicrobial
and fungi cidal properties
Ключевы е слова - антибактериальные полимерные добавки, антибактериальные композиционные
материалы, циклические имиды, антимикробные и фунгицидные свойства

IN TRODUCTION
In recent years, the achievements of polymer
chemistry have taken a wide place in solving vari ous
important problems in medicine, agriculture and other
fields. The presence of antibacterial properties in
polymeric materials (PM) in addition to their physical
– chemi cal and mechanical properties is also an
important issue.
As it is known, the polym er materials are
subjected to biocorrosion under the influence of
microbes and microfungi. Sometimes, the polymers
themselves become microbial centers . Therefore, its
neces sery for polymeric materials to have antimicrobial
protection [1].
Depending on the application area, different
methods are used to obtain antibacterial polymeric
materials. One of these methods is the supplementation
of appropriate a ntibacterial compound s – additionals
into the finished polymer materials during the
processing phase. Thu s, more than 3,000 antibacterial
compounds have been tested in various polymeric
materials [2].
At present, polymer compositions (PC) with
different pr operties have been ob tained by varying the
type and amount of additives included in the PM [3].
Among org anic antipasychics, a wide range of
antiseptics are widely used salicylic and other
derivatives of salicylic acid. It should also be noted that
the com pounds that hold the salicylic group are non -
toxic to human and animal organisms [4]. Alkyl esters
of sal icylic acid have many advantages, such as
antimicrobial substance: poorly soluble in water, highly
antibacterial and inexpensive [5].
In previous stud ies, have reported i n the literature
on unsaturated derivatives of salicylic acid [6] and
cyclic bisimid es [7] and their antibacterial composition

Национальная ассоциация ученых (НАУ) # 50 , 201 9 57
materials. In the presented article, the antibacterial
properties of maleimide derivatives of aminosalicylic
acids were comparati vely characteristic.
The purpose of this article is to preparation and
study the ant ibacterial polymer composition materials
from these compounds.
EXPERIMENTAL PART
The disc -diffusion method was used to study the
initial antibacterial and antifungal effe cts of the
presented substances [8].
To study these properties, Staphylococcus aureu s
(gold staphylococci), Gram -negative bacteria,
Escherichia coli (Gastrointestinal Bacteroids),
Pigmentus, as representative of Gram positive bacteria,
the main contributor s to purely inflammatory processes
commonly used as culturas; Candida albicans, Cand ida
albicans (Candida), representative of fungus, Bacillus
anthracoides as representative of sporadic gram -
positive bacteria, Klebsiella pneumonia as
rep resentative of caps ular bacteria.
Disc -diffusion method is prepared from
microorganisms daily culture, 1 ml microbe cell
suspension per 1 ml, which is less than 1 mL of
standard microbial culture on a sterile physiological
solution and dissolved in 1 ml. i s delivered. Separa te
microbial suspension is then poured into the Petri
bowls, with the APA (Meat -Pept on Flow) and Saburo
White. The bowls are moved so that the suspension is
spread evenly. The residual suspension is then absorbed
through a pipette and th rown into the disin fectant. The
cups are kept at 37°C for 10 min. to allow the solution
to dry slightly . The cups are then removed from the
thermostat and sterilized discs preheated on sterile
essential oil for 3 to 5 minutes are placed on the surface
of t he microbial germin ating environment, with the
pinset gently pressed so that the disks are well warmed.
Subsequently, the PPA bowls are placed at a
temperature of 37°C and the Saburo plantings at a
temperature of 28°C. As the disks are dispersed, the
subst ance injected diffe rs into the agar and kills the
germ. After 24 -48 hours the bowls are removed from
the thermostat and the results are recorded.
RESULTS AND DISCUSSION
The results are presented in Table 1.
Table 1
Antimicrobial effects of synthesized co mpounds and control s
Test
-cultures
ortho -salicylic maleinimide meta -salicylic maleinimide para -salicy lic maleinimide
Sterile
distilled
water -
soluble
Concentrator
(with sterile
distilled water
immersion
disc)
Sterile
distilled
water -
soluble
Concentrator
(with sterile
distilled water
immersion
disc)
Sterile
distilled
water -
soluble
Concentrator
(with ster ile
distilled water
immersion
disc)
St.aureus 3 mm 16 mm 0 mm 6 mm 0 mm 5 mm
E.coli 2 mm 20 mm 0 mm 8 mm 0 mm 6 mm
Ps.aeruginoza 0 mm 5 mm 0 mm 0 mm 0 m m 0 mm
C.albicans 8 mm 25 mm 6 mm 24 mm 5 mm 28mm
Kl.pneumoniae 3 mm 18 mm 0mm 10 mm 3 mm 3 mm
B.a nthracoides 0 mm 21 mm 0mm 20 mm 0 mm 19 mm
Kontrol(etanol) 3 mm 3mm 3 mm 3mm 3 mm 3mm
Note: Figures show the diameter of microbial zones in mm. All expe riments were repeated 3 -5 times.

Conclusion
Studies showed that, distributed ortho -, meta -, and
para -salicylimide derivatives exhibited active effect on
selected microorganism cultures in concentrate, on the
other hand, showed weak antimicrobial activity in
sterile distilled water. As a comparison, all thre e
compound were more effective on fungal cells.

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